Background: The COVID-19 pandemic caused multiple stressors that may lead to symptoms of adjustment disorder.
Objective: We longitudinally examined relationships between risk and protective factors, pandemic-related stressors and symptoms of adjustment disorder during the COVID-19 pandemic, as well as whether these relationships differed by the time of assessment.
Method: The European Society for Traumatic Stress Studies (ESTSS) ADJUST Study included N = 15,169 participants aged 18 years and above. Participants from 11 European countries were recruited and screened three times at 6-month intervals from June 2020 to January 2022. Associations between risk and protective factors (e.g. gender), stressors (e.g. fear of infection), and symptoms of adjustment disorder (AjD, ADNM-8) and their interaction with time of assessment were examined using mixed linear regression.
Results: The following predictors were significantly associated with higher AjD symptom levels: female or diverse gender; older age; pandemic-related news consumption >30 min a day; a current or previous mental health disorder; trauma exposure before or during the pandemic; a good, satisfactory or poor health status (vs. very good); burden related to governmental crisis management and communication; fear of infection; restricted social contact; work-related problems; restricted activity; and difficult housing conditions. The following predictors were associated with lower AjD levels: self-employment or retirement; working in healthcare; and face-to-face contact ≥ once a week with loved ones or friends. The effects of the following predictors on AjD symptoms differed by the time of assessment in the course of the pandemic: a current or previous mental disorder; burden related to governmental crisis management; income reduction; and a current trauma exposure.
Conclusions: We identified risk factors and stressors predicting AjD symptom levels at different stages of the pandemic. For some predictors, the effects on mental health may change at different stages of a pandemic.
View lessBackground
Suicidal ideation is a major concern in clinical practice. Yet, little is known about prevalence rates of suicidal ideation in patients undergoing outpatient psychotherapeutic treatment. Therefore, the aim of the current study is to assess the prevalence of suicidal ideation in a large sample of psychotherapy outpatients in Germany. The data analyzed in this study is taken from the KODAP-project on the coordination of data collection and analysis at German university-based research and training outpatient clinics for psychotherapy.
Methods
A total of N = 10,357 adult outpatients (64.4 % female; age: M(SD) = 35.94 (13.54), range: 18–92 years of age) starting cognitive-behavioral therapy at one of 27 outpatient clinics in Germany were included in the current study. Prevalence of suicidal ideation was assessed with the Suicide Item (Item 9) of the Beck-Depression Inventory II.
Results
Suicidal ideation was reported by 36.7 % (n = 3795) of the participants. Borderline Personality Disorder, Posttraumatic Stress Disorder, and recurrent Major Depression were the diagnoses most strongly associated with the presence and severity of suicidal ideation.
Limitation
Suicide ideation was assessed only with the respective item of the Beck Depression Inventory II.
Conclusion
Suicidal ideation is very common among adult patients who start psychotherapy in Germany. A well-founded knowledge of risk assessment in suicidal patients and suicide-specific treatment options is therefore highly relevant.
View lessThis work reviews possible signatures and potential detectability of present-day volcanically emitted material in the atmosphere of Venus. We first discuss the expected composition of volcanic gases at present time, addressing how this is related to mantle composition and atmospheric pressure. Sulfur dioxide, often used as a marker of volcanic activity in Earth’s atmosphere, has been observed since late 1970s to exhibit variability at the Venus’ cloud tops at time scales from hours to decades; however, this variability may be associated with solely atmospheric processes. Water vapor is identified as a particularly valuable tracer for volcanic plumes because it can be mapped from orbit at three different tropospheric altitude ranges, and because of its apparent low background variability. We note that volcanic gas plumes could be either enhanced or depleted in water vapor compared to the background atmosphere, depending on magmatic volatile composition. Non-gaseous components of volcanic plumes, such as ash grains and/or cloud aerosol particles, are another investigation target of orbital and in situ measurements. We discuss expectations of in situ and remote measurements of volcanic plumes in the atmosphere with particular focus on the upcoming DAVINCI, EnVision and VERITAS missions, as well as possible future missions.
View lessContamination of water bodies is a global environmental and human health issue. Conventional water treatment systems cannot efficiently eliminate organic contaminants, particularly drugs. Photocatalysis is a promising, environmentally friendly oxidation process for the removal of such compounds. A key point is the choice of material to be used as photocatalyst. Here, TiO2/CaTiO3/Cu2O/Cu composites were fabricated by adding different amounts (x) of graphene oxide (GO) (x wt% = 1, 3, and 5 %) to CaCu3Ti4O12 powder using the solid-state synthesis method. The produced pellets were sintered under inert nitrogen atmosphere at 1100 °C for 3 h. X-ray diffraction analysis showed that the Cu metal amount was increased upon GO addition, and the UV–Vis diffuse reflectance spectroscopy showed that the spectral response was extended to the visible range. Then, high performance liquid chromatography assessment of paracetamol degradation by a photocatalytic cell using TiO2/CaTiO3/Cu2O/Cu composites with different GO amounts showed that the removal efficiency was increased upon introduction of 0.5 mM peroxymonosulfate (PMS) as active component to generate radical dotSO4‾ radicals. After 3 h under visible light, 96 % of 10 ppm paracetamol was degraded by the composite with 3 % of GO (1 cm2 surface photocatalyst) compared with 50 % by the composite without GO in the same experimental conditions (PMS in 210 mL of aqueous solution). Free radical trapping and the acute toxicity of potential degradation by-products were also investigated. Our results indicate that TiO2/CaTiO3/Cu2O/Cu with 3 % GO displays long-term stability and durability for the photocatalytic removal of pharmaceutical pollutants from wastewater.
View lessMuch attention has been paid to dynamical simulation and quantum machine learning (QML) independently as applications for quantum advantage, while the possibility of using QML to enhance dynamical simulations has not been thoroughly investigated. Here we develop a framework for using QML methods to simulate quantum dynamics on near-term quantum hardware. We use generalization bounds, which bound the error a machine learning model makes on unseen data, to rigorously analyze the training data requirements of an algorithm within this framework. Our algorithm is thus resource efficient in terms of qubit and data requirements. Furthermore, our preliminary numerics for the XY model exhibit efficient scaling with problem size, and we simulate 20 times longer than Trotterization on IBMQ-Bogota.
View lessBackground
Considering the growing demand for plant trait data and taking into account the lack of trait data from Eastern Europe, especially from its steppic region, we launched a new Ukrainian Plant Trait Database (UkrTrait v. 1.0) aiming at collecting all the available plant trait data from Ukraine.
To facilitate further use of this database, we linked the trait terminology to the TRY Plant Trait Database, Thesaurus of Plant Characteristics (TOP) and Plant Trait Ontology (TO). For taxa names, we provide the crosswalks between the Ukrainian checklist and international sources, i.e. GBIF Backbone Taxonomy, World Checklist of Vascular Plants (World Checklist of Vascular Plants (World Checklist of Vascular Plants (WCVP), World Flora Online (WFO) and Euro+Med PlantBase. We aim to integrate our data into the relevant global (TRY Plant Trait Database) and pan-European (FloraVeg.EU) databases. The current version of the database is freely available at the Zenodo repository and will be updated in the future.
New information
Until now, plant traits for the Ukrainian flora were scattered across literature, often focusing on single species and written mainly in Ukrainian. Additionally, many traits were in grey literature or remained non-digitised, which rendered them inaccessible to the global scientific community. Addressing this gap, our Ukrainian Plant Trait Database (UkrTrait v. 1.0) represents a significant step forward. We compiled and digitised plant traits from local Ukrainian literature sources. Furthermore, we performed our own field and laboratory measurements of various plant traits that were not previously available in literature. In the current version of the UkrTrait, we focus on vascular plant species that are absent from the other European trait databases, with emphasis on species that are representative for the steppe vegetation. Traits assembled from literature include life span (annuals, biennials, perennials), plant height, flowering period (flowering months), life form (by Raunkiaer), plant growth form and others. Our own measured traits include seed mass, seed shape, leaf area, leaf nitrogen concentration and leaf phosphorus concentration. The current version, i.e. UkrTrait v. 1.0, comprises digitised literature data of 287,948 records of 75 traits for 6,198 taxa and our own trait measurements of 2,390 records of 12 traits for 388 taxa.
View lessHumans and many other animal species act in ways that benefit others. Such prosocial behaviour has been studied extensively across a range of disciplines over the last decades, but findings to date have led to conflicting conclusions about prosociality across and even within species. Here, we present a conceptual framework to study the proximate regulation of prosocial behaviour in humans, non-human primates and potentially other animals. We build on psychological definitions of prosociality and spell out three key features that need to be in place for behaviour to count as prosocial: benefitting others, intentionality, and voluntariness. We then apply this framework to review observational and experimental studies on sharing behaviour and targeted helping in human children and non-human primates. We show that behaviours that are usually subsumed under the same terminology (e.g. helping) can differ substantially across and within species and that some of them do not fulfil our criteria for prosociality. Our framework allows for precise mapping of prosocial behaviours when retrospectively evaluating studies and offers guidelines for future comparative work.
View lessFault zones exhibit 3D variable thickness, a feature that remains inadequately explored, particularly with regard to the impact on fluid flow. Upon analyzing an analytic solution, we examine 3D thermal-hydraulic (TH) dynamical models through a benchmark experiment, which incorporates a fault zone with thickness variations corresponding to realistic orders of magnitude. The findings emphasize an area of interest where vigorous convection drives fluid flow, resulting in a temperature increase to 150°C at a shallow depth of 2.7 km in the thickest sections of the fault zone. Moreover, by considering various tectonic regimes (compressional, extensional, and strike-slip) within 3D thermal-hydraulic-mechanical (THM) models and comparing them to the benchmark experiment, we observe variations in fluid pressure induced by poroelastic forces acting on fluid flow within the area of interest. These tectonic-induced pressure changes influence the thermal distribution of the region and the intensity of temperature anomalies. Outcomes of this study emphasize the impact of poroelasticity-driven forces on transfer processes and highlight the importance of addressing fault geometry as a crucial parameter in future investigations of fluid flow in fractured systems. Such research has relevant applications in geothermal energy, CO2 storage, and mineral deposits.
View lessQuantum embedding (QE) methods such as the ghost Gutzwiller approximation (gGA) offer a powerful approach to simulating strongly correlated systems, but come with the computational bottleneck of computing the ground state of an auxiliary embedding Hamiltonian (EH) iteratively. In this work, we introduce an active learning (AL) framework integrated within the gGA to address this challenge. The methodology is applied to the single-band Hubbard model and results in a significant reduction in the number of instances where the EH must be solved. Through a principal component analysis (PCA), we find that the EH parameters form a low-dimensional structure that is largely independent of the geometric specifics of the systems, especially in the strongly correlated regime. Our AL strategy enables us to discover this low-dimensionality structure on the fly, while leveraging it for reducing the computational cost of gGA, laying the groundwork for more efficient simulations of complex strongly correlated materials.
View lessIn this work we study the global solvability of moisture dynamics with phase changes for warm clouds. We thereby in comparison to previous studies (Hittmeir et al. in Nonlinearity 30:3676–3718, 2017) take into account the different gas constants for dry air and water vapor as well as the different heat capacities for dry air, water vapor and liquid water, which leads to a much stronger coupling of the moisture balances and the thermodynamic equation. This refined thermodynamic setting has been demonstrated to be essential, e.g. in the case of deep convective cloud columns in Hittmeir and Klein (Theoret Comput Fluid Dyn 32(2):137–164, 2017). The more complicated structure requires careful derivations of sufficient a priori estimates for proving global existence and uniqueness of solutions.
View lessObjectives
The objective of the present study was to examine the diversity of Staphylococcus aureus from mastitis milk samples of cows in Rwanda.
Methods
A total of 1080 quarter milk samples from 279 dairy cows were collected in 80 different farms from all five provinces of Rwanda. In total, 135 S. aureus isolates were obtained and subjected to genotyping (spa typing, DNA microarray, whole-genome sequencing (WGS)), antimicrobial susceptibility testing (AST) and phenotypic profiling by Fourier Transform Infrared (FTIR) spectroscopy (including capsular serotyping).
Results
Resistance to penicillin and/or tetracycline was most frequently observed. Ten sequence types (STs) (ST1, ST151, ST152, ST5477, ST700, ST7110, ST7983, ST7984, ST8320, ST97) belonging to seven clonal complexes (CCs) (CC1, CC130, CC152, CC3591, CC3666, CC705, CC97) were detected. The Panton–Valentine leukocidin (PVL) genes (lukF-PV/lukS-PV), the bovine leukocidin genes (lukM/lukF-P83) and the human and bovine toxic shock syndrome toxin gene tst-1 variants were detected. FTIR-based capsular serotyping showed CC-specific differences. Most CC97 (cap5 allele) isolates were primarily nonencapsulated (82%), whereas isolates of CC3591 and CC3666 (cap8 allele) were mostly encapsulated (86.4% and 57.8%, respectively). Our results underline the widespread global distribution of cattle-adapted CC97.
Conclusion
The presence of CC3591 and CC3666 in bovine mastitis suggests an important role in cattle health and dairy production in Rwanda. The results of the present study support the need for a rigorous One-Health Surveillance program of the bovine–human interface.
View lessMicroplastics (MPs), including conventional hard-to-biodegrade petroleum-based and faster biodegradable plant-based ones, impact soil structure and microbiota in turn affecting the biodiversity and functions of terrestrial ecosystems. Herein, we investigated the effects of conventional and biodegradable MPs on aggregate distribution and microbial community composition in microhabitats at the aggregate scale. Two MP types (polyethylene (PE) and polylactic acid (PLA) with increasing size (50, 150, and 300 μm)) were mixed with a silty loam soil (0–20 cm) at a ratio of 0.5 % (w/w) in a rice–wheat rotation system in a greenhouse under 25 °C for one year. The effects on aggregation, bacterial communities and their co-occurrence networks were investigated as a function of MP aggregate size. Conventional and biodegradable MPs generally had similar effects on soil aggregation and bacterial communities. They increased the proportion of microaggregates from 17 % to 32 %, while reducing the macroaggregates from 84 % to 68 %. The aggregate stability decreased from 1.4 mm to 1.0–1.1 mm independently of MP size due to the decline in the binding agents gluing soil particles (e.g., microbial byproducts and proteinaceous substances). MP type and amount strongly affected the bacterial community structure, accounting for 54 % of the variance. Due to less bioavailable organics, bacterial community composition within microaggregates was more sensitive to MPs addition compared to macroaggregates. Co-occurrence network analysis revealed that MPs exacerbated competition among bacteria and increased the complexity of bacterial networks. Such effects were stronger for PE than PLA MPs due to the higher persistence of PE in soils. Proteobacteria, Bacteroidetes, Chloroflexi, Actinobacteria, and Gemmatimonadetes were the keystone taxa in macroaggregates, while Actinobacteria and Chloroflexi were the keystone taxa in microaggregates. Proteobacteria, Actinobacteria, and Chloroflexi were the most sensitive bacteria to MPs addition. Overall, both conventional and biodegradable MPs reduced the portion of large and stable aggregates, altering bacterial community structures and keystone taxa, and consequently, the functions.
View lessChangbaishan volcano (China/North Korea) is one of the most active and hazardous volcanic centers in Northeast Asia. Despite decades of intensive research, the eruption history of this stratovolcano remains poorly constrained. One of the major puzzles is the timing of the eruption that produced the Tianwen Yellow Pumice (TYP) deposit at the caldera rim. Here we identify a new cryptotephra layer in sediment core 13PT-P4 from the East Sea. Grain-specific major, minor, and trace element analyses of glass shards allow a clear correlation of this distal tephra to the proximal TYP deposit of Changbaishan. Age-depth modeling using radiocarbon (14C) dates of sediment bulk organic fractions and other tephrochronological markers from the sediment sequence constraints the age of the cryptotephra and thus the TYP eruption to 29,948–29,625 cal yr BP (95.4% confidence interval). Our findings lead to a revision of the history of Changbaishan explosive activity, and show that the volcano has been particularly active during ca. 51–24 ka BP in the last 100 ka. Using high resolution palaeo-proxy records, we find the TYP tephra almost coeval with regional to hemispheric-scale climatic changes known as Heinrich Event 3 (H3). With its precise age determination and wide geographic dispersion, the tephra offers a key isochron for dating records of past climatic changes and addressing the phasing relationships in environmental response to H3 across East Asia.
View lessTopological insulators and superconductors support extended surface states protected against the otherwise localizing effects of static disorder. Specifically, in the Wigner-Dyson insulators belonging to the symmetry classes A, AI, and AII, a band of extended surface states is continuously connected to a likewise extended set of bulk states forming a “bridge” between different surfaces via the mechanism of spectral flow. In this work we show that this mechanism is absent in the majority of non-Wigner-Dyson topological superconductors and chiral topological insulators. In these systems, there is precisely one point with granted extended states, the center of the band, E=0. Away from it, states are spatially localized, or can be made so by the addition of spatially local potentials. Considering the three-dimensional insulator in class AIII and winding number ν=1 as a paradigmatic case study, we discuss the physical principles behind this phenomenon, and its methodological and applied consequences. In particular, we show that low-energy Dirac approximations in the description of surface states can be treacherous in that they tend to conceal the localizability phenomenon. We also identify markers defined in terms of Berry curvature as measures for the degree of state localization in lattice models, and back our analytical predictions by extensive numerical simulations. A main conclusion of this work is that the surface phenomenology of non-Wigner-Dyson topological insulators is a lot richer than that of their Wigner-Dyson siblings, extreme limits being spectrumwide quantum critical delocalization of all states versus full localization except at the E=0 critical point. As part of our study we identify possible experimental signatures distinguishing between these different alternatives in transport or tunnel spectroscopy.
View lessTracking livestock abortion patterns over time and across factors such as species and agroecological zones (AEZs) could inform policies to mitigate disease emergence, zoonoses risk, and reproductive losses. We conducted a year-long population-based active surveillance of livestock abortion between 2019 and 2020, in administrative areas covering 52% of Kenya’s landmass and home to 50% of Kenya’s livestock. Surveillance sites were randomly selected to represent all AEZs in the country. Local animal health practitioners electronically transmitted weekly abortion reports from each ward, the smallest administrative unit, to a central server, using a simple short messaging service (SMS). Data were analyzed descriptively by administrative unit, species, and AEZ to reveal spatiotemporal patterns and relationships with rainfall and temperature. Of 23,766 abortions reported in all livestock species, sheep and goats contributed 77%, with goats alone contributing 53%. Seventy-seven per cent (n = 18,280) of these abortions occurred in arid and semi-arid lands (ASALs) that primarily practice pastoralism production systems. While spatiotemporal clustering of cases was observed in May-July 2019 in the ASALs, there was a substantial seasonal fluctuation across AEZs. Kenya experiences high livestock abortion rates, most of which go unreported. We recommend further research to document the national true burden of abortions. In ASALs, studies linking pathogen, climate, and environmental surveillance are needed to assign livestock abortions to infectious or non-infectious aetiologies and conducting human acute febrile illnesses surveillance to detect any links with the abortions.
View lessThis introduction outlines the contemporary emergence of new forms of informal crisis-related care, which both complement and contradict classical forms of humanitarian assistance. The introduction traces the spread, blurring, and differentiation of novel forms of non-state assistance and support against the backdrop of increasingly widespread criticism of large-scale international aid. Tackling regimes of care beyond the exceptionality of a crisis notion, the introduction then summarizes how the three contributions and the commentary to this theme section employ the lens of affect for exploring how these highly intersubjective forms of encounter are experienced, performed, and reflected on.
View lessIntroduction
Brucellosis is a febrile zoonosis occurring among high-risk groups such as livestock keepers and abattoir workers and is a public health priority in Uganda. The technical complexities of bacteriological and molecular methods make serological approaches the cornerstone of diagnosis of human brucellosis in resource limited settings. Therefore, proper application and interpretation of serological tests is central to achieve a correct diagnosis.
Materials and methods
We conducted a cross-sectional study to estimate the seroprevalence and factors associated with anti-Brucella antibodies among slaughterhouse workers processing ruminants and pigs in three regions of the country with serial testing using a combination of the Rose Bengal Test (RBT) and the BrucellaCapt test. An authorized clinician collected 543 blood samples from consenting abattoir workers as well as attribute medical and social demographic data. Univariable and multivariable logistic regression were used to determine factors associated with anti-Brucella sero-positivity.
Results and discussion
The sero-prevalence among ruminant slaughterhouse workers ranged from 7.3% (95% CI: 4.8–10.7) using BrucellaCapt to 9.0% (95% CI: 6.3–12.7) using RBT. Slaughterhouse workers from the Eastern regions (AOR = 9.84, 95%CI 2.27–69.2, p = 0.006) and those who graze animals for alternative income (AOR = 2.36, 95% CI: 1.91–6.63, p = 0.040) were at a higher risk of exposure to Brucella. Similarly, those who wore Personal Protective Equipment (AOR = 4.83, 95%CI:1.63–18.0, p = 0.009) and those who slaughter cattle (AOR = 2.12, 95%CI: 1.25–6.0, p = 0.006) were at a higher risk of exposure to Brucella. Those who slaughter small ruminants (AOR = 1.54, 95%CI: 1.32–4.01, p = 0.048) were also at a higher risk of exposure to Brucella.
Conclusions and recommendations
Our study demonstrates the combined practical application of the RBT and BrucellaCapt in the diagnosis of human brucellosis in endemic settings. Both pharmaceutical (e.g., routine testing and timely therapeutic intervention), and non-pharmaceutical (e.g., higher index of suspicion of brucellosis when investigating fevers of unknown origin and observation of strict abattoir hygiene) countermeasures should be considered for control of the disease in high-risk groups.
View lessTo trace Critical Zone processes and to quantify Li fluxes from one Critical Zone compartment into another, we investigated the Li concentration and isotopic composition (δ7Li) of time-series water samples (including subsurface flow, groundwater and creek water), vegetation, bedrock (including separated minerals from bedrock), and regolith (including exchangeable fraction and clay-sized fraction of regolith) in a temperate forested headwater catchment in the Black Forest, Conventwald, Germany. Our estimation of the Li budget shows that atmospheric deposition and biological processes played minor roles in the Li cycle relative to chemical weathering. All water samples (δ 7Li value of 6.5 to 20.4 ‰) were enriched in 7Li compared to bedrock (-1.3 ‰) and regolith (∼-1.7 ‰), but δ7Li differed between water pathways: δ7Li variations in subsurface flow, creek water and groundwater were controlled by conservative mixing, exchangeable pool buffering and Li incorporation/adsorption, respectively. Fractionated heavy Li isotopes in water samples resulted from the formation of secondary solids which preferentially incorporated 6Li, with the separated clay-sized fraction of the regolith exhibiting more negative δ7Li values (-5.4 to −3.5 ‰) than the bulk regolith (∼-1.7 ‰). However, Li in secondary solids only accounted for 8 ± 6 % of the total Li hosted in bulk regolith, and consequently δ7Li in soil did not differ significantly from δ 7Li in bedrock. This is unexpected considering water is continuously removing 7Li in preference over 6Li from regolith. Mass balance calculations applied at the catchment scale point to an irreconcilable imbalance with our data. On one hand, the regolith’s δ7Li values are not negative enough to balance the 7Li export by river water, and on the other hand Li in the riverine dissolved load only accounts for ∼ 30 % of the Li solubilized from regolith. Therefore, we suggest that there might be a “hidden export pathway” for Li at our site, possibly subsurface removal of fine particles enriched in 6Li. In light of increasingly frequent observations of such isotopic imbalances in the Critical Zone this phenomenon deserves increased attention.
View lessWe present a robust radiocarbon (14C) chronology for burials at Sakhtysh, in European Russia, where nearly 180 inhumations of Lyalovo and Volosovo pottery-using hunter-gatherer-fishers represent the largest known populations of both groups. Past dating attempts were restricted by poor understanding of dietary 14C reservoir effects (DREs). We developed a DRE correction approach that uses multiple linear regression of differences in 14C, δ13C, and δ15N between bones and teeth of the same individuals to predict DREs of up to approximately 900 years. Our chronological model dates Lyalovo burials to the early fifth millennium BCE, and Volosovo burials to the mid-fourth to early third millennium. It reveals a change in the subsistence economy at approximately 3300 BCE, coinciding with a reorientation of trade networks, and dates the final burial to the early Fatyanovo period, the regional expression of the Yamnaya/Corded Ware expansion. Our approach is applicable when freshwater 14C reservoir effects are poorly constrained and grave goods cannot be dated directly.
View lessIntroduction
Contrast-associated acute kidney injury (CA-AKI) is characterized as a loss of renal function following radiological contrast media administration. While all contrast media induce variable changes in microvascular endothelial cells in vitro, only few studies report clinical significance of their findings. A comprehensive assessment of the effect of iodinated contrast media on the renal function in vitro and in vivo is essential. The aim of our study was to morphometrically quantify the effect of two different contrast media (Iobitridol and Iodixanol) on vascular endothelial capillaries in vitro and to analyze their effect on the renal function of patients who underwent cardiac catheterization including the intra-arterial administration of contrast media, by measuring serum creatinine concentration (SCr), a byproduct of muscle metabolism, primarily excreted by the kidneys. Our hypothesis suggests that conducting a qualitative comparison of both outcomes will enable identification of differences and similarities between in vitro and in vivo exposure.
Material and methods
In vitro, co-cultures of human dermal fibroblasts and human dermal microvascular endothelial cells forming capillary beds were exposed to a mixture of phosphate buffered saline and either Iobitridol, Iodixanol, or one of their supplements EDTA or Trometamol for 1.5 or 5 min. Negative control co-cultures were exposed exclusively to phosphate buffered saline. Co-cultures were either directly fixed or underwent a regeneration time of 1, 3 or 7 days. An artificial intelligence software was trained for detection of labeled endothelial capillaries (CD31) on light microscope images and measurements of morphometric parameters. In vivo, we retrospectively analyzed data from patients who underwent intra-arterial administration of contrast media and for whom SCr values were available pre- and post-contrast exposition (1, 3, and 7 days following procedure). Temporal development of SCr and incidence of CA-AKI were assessed. Both exposure types were qualitatively compared.
Results
In vitro, Iobitridol, Iodixanol and EDTA induced a strong decrease of two morphometric parameters after 3 days of regeneration. In vivo, a significant increase of SCr and incidence of CA-AKI was observed 3 days following procedure in the post-contrast media patients. No difference was observed between groups.
Discussion
Two of the morphometric parameters were inversely proportional to the SCr of the patients. If the endothelial damages observed in vitro occur in vivo, it may result in renal hypoxia, inducing a loss of kidney function clinically translated into an increase of SCr. Further development of our in vitro model could allow closer replication of the internal structure of a kidney and bridge the gap between in vitro studies and their clinical findings.
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