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Jim Hyde and the ENDOR Connection
Möbius, Klaus ;  Lubitz, Wolfgang ;  Savitsky, Anton

HaupttitelJim Hyde and the ENDOR Connection
TitelzusatzA Personal Account
AutorMöbius, Klaus
AutorLubitz, Wolfgang
AutorSavitsky, Anton
Seitenzahl35 Seiten
DDC530 Physik
Auch erschienen inApplied Magnetic Resonance. - 48 (2017), 11-12, S.1149-1183
ZusammenfassungIn this minireview, we report on our year-long EPR work, such as electron–nuclear double resonance (ENDOR), pulse electron double resonance (PELDOR) and ELDOR-detected NMR (EDNMR) at X-band and W-band microwave frequencies and magnetic fields. This report is dedicated to James S. Hyde and honors his pioneering contributions to the measurement of spin interactions in large (bio)molecules. From these interactions, detailed information is revealed on structure and dynamics of macromolecules embedded in liquid-solution or solid-state environments. New developments in pulsed microwave and sweepable cryomagnet technology as well as ultra-fast electronics for signal data handling and processing have pushed the limits of EPR spectroscopy and its multi-frequency extensions to new horizons concerning sensitivity of detection, selectivity of molecular interactions and time resolution. Among the most important advances is the upgrading of EPR to high magnetic fields, very much in analogy to what happened in NMR. The ongoing progress in EPR spectroscopy is exemplified by reviewing various multi-frequency electron–nuclear double-resonance experiments on organic radicals, light-generated donor–acceptor radical pairs in photosynthesis, and site-specifically nitroxide spin-labeled bacteriorhodopsin, the light-driven proton pump, as well as EDNMR and ENDOR on nitroxides. Signal and resolution enhancements are particularly spectacular for ENDOR, EDNMR and PELDOR on frozen-solution samples at high Zeeman fields. They provide orientation selection for disordered samples approaching single-crystal resolution at canonical g-tensor orientations—even for molecules with small g-anisotropies. Dramatic improvements of EPR detection sensitivity could be achieved, even for short-lived paramagnetic reaction intermediates. Thus, unique structural and dynamic information is revealed that can hardly be obtained by other analytical techniques. Micromolar concentrations of sample molecules have become sufficient to characterize stable and transient reaction intermediates of complex molecular systems—offering exciting applications for physicists, chemists, biochemists and molecular biologists.
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Fachbereich/EinrichtungFB Physik
Arbeitsbereich/InstitutInstitut für Experimentalphysik
Erscheinungsjahr2017
Dokumententyp/-SammlungenWissenschaftlicher Artikel
SpracheEnglisch
RechteCreative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Erstellt am03.11.2017 - 00:00:00
Letzte Änderung03.11.2017 - 00:00:00
 
Statische URLhttp://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000028432
SEPID60445
ISSN0937-9347
DOI10.1007/s00723-017-0959-9
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