Objekt-Metadaten

SERPINB2 is a novel TGFβ-responsive lineage fate determinant of human bone marrow stromal cells
Elsafadi, Mona ;  Manikandan, Muthurangan ;  Atteya, Muhammad ;  Abu Dawud, Raed ;  Almalki, Sami ;  Kaimkhani, Zahid Ali ;  Aldahmash, Abdullah ;  Alajez, Nehad M. ;  Alfayez, Musaad ;  Kassem, Moustapha ;  Mahmood, Amer

HaupttitelSERPINB2 is a novel TGFβ-responsive lineage fate determinant of human bone marrow stromal cells
TitelvarianteSERPINB2 is a novel TGF(beta)-responsive lineage fate determinant of human bone marrow stromal cells
AutorElsafadi, Mona
AutorManikandan, Muthurangan
AutorAtteya, Muhammad
AutorAbu Dawud, Raed
AutorAlmalki, Sami
AutorKaimkhani, Zahid Ali
AutorAldahmash, Abdullah
AutorAlajez, Nehad M.
AutorAlfayez, Musaad
AutorKassem, Moustapha
AutorMahmood, Amer
Seitenzahl15 S.
Freie SchlagwörterGene expression analysis; Laboratory techniques and procedures; Stem-cell differentiation
DDC610 Medizin und Gesundheit
Auch erschienen inScientific Reports. - 7 (2017), Artikel Nr. 10797
ZusammenfassungTGF-β1, a multifunctional regulator of cell growth and differentiation, is the most abundant bone matrix growth factor. During differentiation of human bone stromal cells (hBMSCs), which constitute bone marrow osteoblast (OS) and adipocyte (AD) progenitor cells, continuous TGF-β1 (10 ng/ml) treatment enhanced OS differentiation as evidenced by increased mineralised matrix production. Conversely, pulsed TGF-β1 administration during the commitment phase increased mature lipid-filled adipocyte numbers. Global gene expression analysis using DNA microarrays in hBMSCs treated with TGF-β1 identified 1587 up- and 1716 down-regulated genes in OS-induced, TGF-β1-treated compared to OS-induced hBMSCs (2.0 fold change (FC), p < 0.05). Gene ontology (GO) analysis revealed enrichment in ‘osteoblast differentiation’ and ‘skeletal system development-associated’ genes and up-regulation of several genes involved in ‘osteoblastic-differentiation related signalling pathways’. In AD-induced, TGF-β1-treated compared to AD-induced hBMSCs, we identified 323 up- and 369 down-regulated genes (2.0 FC, p < 0.05) associated with ‘fat cell differentiation’, ‘fatty acid derivative biosynthesis process’, ‘fatty acid derivative metabolic process’, and ‘inositol lipid-mediated’. Serpin peptidase inhibitor, clade B (ovalbumin), member 2 (SERPINB2) was down-regulated 3-fold in TGF-β1-treated hBMSCs. siRNA-mediated SERPINB2 inhibition enhanced OS and AD differentiation. Thus, TGF-β signalling is important for hBMSC OS and AD differentiation and SERPINB2 is a TGF-β-responsive gene that plays a negative regulatory role in hBMSC differentiation.
Dokumente
PDF-Datei von FUDOCS_document_000000028308
Falls Ihr Browser eine Datei nicht öffnen kann, die Datei zuerst herunterladen und dann öffnen.
 
Fachbereich/EinrichtungMedizinische Fakultät Charité - Universitätsmedizin Berlin
Erscheinungsjahr2017
Dokumententyp/-SammlungenWissenschaftlicher Artikel
SpracheEnglisch
RechteCreative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Anmerkungen des AutorsDer Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
Erstellt am13.10.2017 - 12:11:40
Letzte Änderung13.10.2017 - 12:12:37
 
Statische URLhttp://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000028308
DOI10.1038/s41598-017-10983-x
Zugriffsstatistik
 

LOADING...