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Cardiovascular magnetic resonance feature tracking in small animals – a preliminary study on reproducibility and sample size calculation
Lapinskas, Tomas ;  Grune, Jana ;  Zamani, Seyedeh Mahsa ;  Jeuthe, Sarah ;  Messroghli, Daniel ;  Gebker, Rolf ;  Meyborg, Heike ;  Kintscher, Ulrich ;  Zaliunas, Remigijus ;  Pieske, Burkert ;  Stawowy, Philipp ;  Kelle, Sebastian

HaupttitelCardiovascular magnetic resonance feature tracking in small animals – a preliminary study on reproducibility and sample size calculation
AutorLapinskas, Tomas
AutorGrune, Jana
AutorZamani, Seyedeh Mahsa
AutorJeuthe, Sarah
AutorMessroghli, Daniel
AutorGebker, Rolf
AutorMeyborg, Heike
AutorKintscher, Ulrich
AutorZaliunas, Remigijus
AutorPieske, Burkert
AutorStawowy, Philipp
AutorKelle, Sebastian
Seitenzahl9 S.
Freie SchlagwörterCardiovascular magnetic resonance; Feature tracking; Myocardial strain; Small animal model; Reproducibility
DDC610 Medizin und Gesundheit
Auch erschienen inBMC Medical Imaging. - 17 (2017), Artikel Nr. 51
ZusammenfassungBackground

Cardiovascular magnetic resonance feature tracking (CMR-FT) is a novel tissue tracking technique developed for noninvasive assessment of myocardial motion and deformation. This preliminary study aimed to evaluate the observer’s reproducibility of CMR-FT in a small animal (mouse) model and define sample size calculation for future trials.

Methods

Six C57BL/6 J mice were selected from the ongoing experimental mouse model onsite and underwent CMR with a 3 Tesla small animal MRI scanner. Myocardial deformation was analyzed using dedicated software (TomTec, Germany) by two observers. Left ventricular (LV) longitudinal, circumferential and radial strain (EllLAX, EccSAX and ErrSAX) were calculated. To assess intra-observer agreement data analysis was repeated after 4 weeks. The sample size required to detect a relative change in strain was calculated.

Results

In general, EccSAX and EllLAX demonstrated highest inter-observer reproducibility (ICC 0.79 (0.46–0.91) and 0.73 (0.56–0.83) EccSAX and EllLAX respectively). In contrast, at the intra-observer level EllLAX was more reproducible than EccSAX (ICC 0.83 (0.73–0.90) and 0.74 (0.49–0.87) EllLAX and EccSAX respectively). The reproducibility of ErrSAX was weak at both observer levels. Preliminary sample size calculation showed that a small study sample (e.g. ten animals to detect a relative 10% change in EccSAX) could be sufficient to detect changes if parameter variability is low.

Conclusions

This pilot study demonstrates good to excellent inter- and intra-observer reproducibility of CMR-FT technique in small animal model. The most reproducible measures are global circumferential and global longitudinal strain, whereas reproducibility of radial strain is weak. Furthermore, sample size calculation demonstrates that a small number of animals could be sufficient for future trials.
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Fachbereich/EinrichtungMedizinische Fakultät Charité - Universitätsmedizin Berlin
Erscheinungsjahr2017
Dokumententyp/-SammlungenWissenschaftlicher Artikel
SpracheEnglisch
RechteCreative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Anmerkungen des AutorsDer Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
Erstellt am12.10.2017 - 08:02:25
Letzte Änderung12.10.2017 - 08:03:25
 
Statische URLhttp://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000028287
DOI10.1186/s12880-017-0223-7
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