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Molecular Characterization of Barrier Properties in Follicle-Associated Epithelium of Porcine Peyer's Patches Reveals Major Sealing Function of Claudin-4
Radloff, Judith ;  Falchuk, Evgeny L. ;  Markov, Alexander G. ;  Amasheh, Salah

HaupttitelMolecular Characterization of Barrier Properties in Follicle-Associated Epithelium of Porcine Peyer's Patches Reveals Major Sealing Function of Claudin-4
AutorRadloff, Judith
AutorFalchuk, Evgeny L.
AutorMarkov, Alexander G.
AutorAmasheh, Salah
Seitenzahl8 S.
Freie Schlagwörterclaudins; tissue barrier; tight junction; pig intestine; gut-associated lymphoid tissue
DDC570 Biowissenschaften; Biologie
630 Landwirtschaft, Veterinärmedizin
Auch erschienen inFront. Physiol. - 8 (2017), Artikel Nr. 579
ZusammenfassungThe pig represents a preferred model for the analysis of intestinal immunology. However, the barrier of the follicle-associated epithelium (FAE) covering porcine Peyer's patches (PP) has not yet been characterized in detail. This study aimed to perform this characterization in order to pave the way toward an understanding of the functional contribution of epithelial barrier properties in gut immunology. Porcine tissue specimens were taken from the distal small intestine in order to obtain electrophysiological data of PP FAE and neighboring villous epithelium (VE), employing the Ussing chamber technique. Transepithelial resistance (TER) and paracellular fluorescein flux were measured, and tissues were morphometrically compared. In selfsame tissues, expression and localization of major tight junction (TJ) proteins (claudin-1, -2, -3, -4, -5, and -8) were analyzed. PP FAE specimens showed a higher TER and a lower apparent permeability for sodium fluorescein than VE. Immunoblotting revealed an expression of all claudins within both epithelia, with markedly stronger expression of the sealing TJ protein claudin-4 in PP FAE compared with the neighboring VE. Immunohistochemistry confirmed the expression and localization of all claudins in both PP FAE and VE, with stronger claudin-4 abundance in PP FAE. The results are in accordance with the physiological function of the FAE, which strongly regulates and limits antigen uptake determining a mandatory transcellular route for antigen presentation, highlighting the importance of this structure for the first steps of the intestinal immune response. Thus, this study provides detailed insights into the specific barrier properties of the porcine FAE covering intestinal PP, at the interface of intestinal immunology and barriology.
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Fachbereich/EinrichtungFB Veterinärmedizin
Erscheinungsjahr2017
Dokumententyp/-SammlungenWissenschaftlicher Artikel
SpracheEnglisch
RechteCreative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Anmerkungen des AutorsDer Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
Erstellt am25.09.2017 - 10:57:15
Letzte Änderung25.09.2017 - 11:01:07
 
Statische URLhttp://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000028002
DOI10.3389/fphys.2017.00579
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