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Altered Cytokine Expression and Barrier Properties after In Vitro Infection of Porcine Epithelial Cells with Enterotoxigenic Escherichia coli and Probiotic Enterococcus faecium
Kern, Martina ;  Günzel, Dorothee ;  Aschenbach, Jörg R. ;  Tedin, Karsten ;  Bondzio, Angelika ;  Lodemann, Ulrike

HaupttitelAltered Cytokine Expression and Barrier Properties after In Vitro Infection of Porcine Epithelial Cells with Enterotoxigenic Escherichia coli and Probiotic Enterococcus faecium
AutorKern, Martina
AutorGünzel, Dorothee
AutorAschenbach, Jörg R.
AutorTedin, Karsten
AutorBondzio, Angelika
AutorLodemann, Ulrike
Seitenzahl13 Seiten
DDC630 Landwirtschaft, Veterinärmedizin
Auch erschienen inMediators of Inflammation : Vol. 2017
ZusammenfassungThe aim of the present study was to elucidate the effects of the probiotic feed additive Enterococcus faecium NCIMB 10415 (E. faecium) on porcine jejunal epithelial cells (IPEC-J2) during an in vitro challenge with enterotoxigenic Escherichia coli (ETEC). Cells were incubated with E. faecium, ETEC, or both, and the effects on barrier function and structure and intra- and intercellular signaling were determined. Coincubation with E. faecium abolished the ETEC-induced decrease in transepithelial resistance (Rt). No differences were seen in the expression levels of the intercellular connecting tight junction proteins examined. However, for the first time, a reorganization of the monolayer was observed in ETEC-infected cells but not in coincubated cells. ETEC induced an increase in cytotoxicity that was prevented by coincubation, whereas apoptosis rates were not affected by bacterial treatment. ETEC increased the mRNA expression and release of proinflammatory cytokines TNF-α, IL-1α, and IL-6 which could be prevented by coincubation for TNF-α mRNA expression and IL-6 protein. Likewise, cAMP concentrations elevated by ETEC were reduced in coincubated cells. These findings indicate a protective effect of the probiotic E. faecium on inflammatory responses during infection with ETEC.
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Fachbereich/EinrichtungFB Veterinärmedizin
Arbeitsbereich/InstitutInstitut für Veterinär-Physiologie
Erscheinungsjahr2017
Dokumententyp/-SammlungenWissenschaftlicher Artikel
SpracheEnglisch
RechteCreative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Anmerkungen des AutorsGefördert durch die DFG und den Open-Access-Publikationsfonds der Freien Universität Berlin.
Erstellt am26.06.2017 - 12:17:17
Letzte Änderung26.06.2017 - 12:19:01
 
Statische URLhttp://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000027250
DOI10.1155/2017/2748192
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