Objekt-Metadaten

Unity in defence
Doublet, Vincent ;  Poeschl, Yvonne ;  Gogol-Doering, Andreas ;  Alaux, Cedric ;  Annoscia, Desiderato ;  Aurori, Christian ;  Genersch, Elke ;  McMahon, Dino P.

HaupttitelUnity in defence
Titelzusatzhoneybee workers exhibit conserved molecular responses to diverse pathogens
AutorDoublet, Vincent
AutorPoeschl, Yvonne
AutorGogol-Doering, Andreas
AutorAlaux, Cedric
AutorAnnoscia, Desiderato
AutorAurori, Christian
AutorGenersch, Elke
AutorMcMahon, Dino P.
Seitenzahl17 S.
Freie SchlagwörterApis mellifera; Nosema; Varroa destructor; DWV; IAPV; RNA virus; Meta-analysis; Transcriptomics; Co-expression network; Immunity
DDC570 Biowissenschaften; Biologie
Auch erschienen inBMC Genomics. - 18 (2017), Artikel Nr. 207
ZusammenfassungBackground

Organisms typically face infection by diverse pathogens, and hosts are thought to have developed specific responses to each type of pathogen they encounter. The advent of transcriptomics now makes it possible to test this hypothesis and compare host gene expression responses to multiple pathogens at a genome-wide scale. Here, we performed a meta-analysis of multiple published and new transcriptomes using a newly developed bioinformatics approach that filters genes based on their expression profile across datasets. Thereby, we identified common and unique molecular responses of a model host species, the honey bee (Apis mellifera), to its major pathogens and parasites: the Microsporidia Nosema apis and Nosema ceranae, RNA viruses, and the ectoparasitic mite Varroa destructor, which transmits viruses.

Results

We identified a common suite of genes and conserved molecular pathways that respond to all investigated pathogens, a result that suggests a commonality in response mechanisms to diverse pathogens. We found that genes differentially expressed after infection exhibit a higher evolutionary rate than non-differentially expressed genes. Using our new bioinformatics approach, we unveiled additional pathogen-specific responses of honey bees; we found that apoptosis appeared to be an important response following microsporidian infection, while genes from the immune signalling pathways, Toll and Imd, were differentially expressed after Varroa/virus infection. Finally, we applied our bioinformatics approach and generated a gene co-expression network to identify highly connected (hub) genes that may represent important mediators and regulators of anti-pathogen responses.

Conclusions

Our meta-analysis generated a comprehensive overview of the host metabolic and other biological processes that mediate interactions between insects and their pathogens. We identified key host genes and pathways that respond to phylogenetically diverse pathogens, representing an important source for future functional studies as well as offering new routes to identify or generate pathogen resilient honey bee stocks. The statistical and bioinformatics approaches that were developed for this study are broadly applicable to synthesize information across transcriptomic datasets. These approaches will likely have utility in addressing a variety of biological questions.
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Fachbereich/EinrichtungFB Biologie, Chemie, Pharmazie
Erscheinungsjahr2017
Dokumententyp/-SammlungenWissenschaftlicher Artikel
SpracheEnglisch
RechteCreative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Anmerkungen des AutorsDer Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
Erstellt am28.04.2017 - 07:26:03
Letzte Änderung28.04.2017 - 07:35:11
 
Statische URLhttp://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000026919
DOI10.1186/s12864-017-3597-6
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