Dendritic Core-Multishell Nanocarriers in Murine Models of Healthy and Atopic Skin
Radbruch, Moritz ;  Pischon, Hannah ;  Ostrowski, Anja ;  Volz, Pierre ;  Brodwolf, Robert ;  Neumann, Falko ;  Unbehauen, Michael ;  Kleuser, Burkhard ;  Haag, Rainer ;  Ma, Nan ;  Alexiev, Ulrike ;  Mundhenk, Lars ;  Gruber, Achim D.

HaupttitelDendritic Core-Multishell Nanocarriers in Murine Models of Healthy and Atopic Skin
AutorRadbruch, Moritz
AutorPischon, Hannah
AutorOstrowski, Anja
AutorVolz, Pierre
AutorBrodwolf, Robert
AutorNeumann, Falko
AutorUnbehauen, Michael
AutorKleuser, Burkhard
AutorHaag, Rainer
AutorMa, Nan
AutorAlexiev, Ulrike
AutorMundhenk, Lars
AutorGruber, Achim D.
Seitenzahl12 Seiten
Freie SchlagwörterCMS; Skin; Topical treatment; Dermal delivery; Atopic dermatitis; Oxazolone; Fluorescence lifetime imaging microscopy; Nanomaterials; Multi-domain nanoparticles; Penetration enhancement
DDC630 Landwirtschaft, Veterinärmedizin
Auch erschienen inNanoscale Research Letters (2017) 12 : 64
ZusammenfassungDendritic hPG-amid-C18-mPEG core-multishell nanocarriers (CMS) represent a novel class of unimolecular micelles that hold great potential as drug transporters, e.g., to facilitate topical therapy in skin diseases. Atopic dermatitis is among the most common inflammatory skin disorders with complex barrier alterations which may affect the efficacy of topical treatment.

Here, we tested the penetration behavior and identified target structures of unloaded CMS after topical administration in healthy mice and in mice with oxazolone-induced atopic dermatitis. We further examined whole body distribution and possible systemic side effects after simulating high dosage dermal penetration by subcutaneous injection.

Following topical administration, CMS accumulated in the stratum corneum without penetration into deeper viable epidermal layers. The same was observed in atopic dermatitis mice, indicating that barrier alterations in atopic dermatitis had no influence on the penetration of CMS. Following subcutaneous injection, CMS were deposited in the regional lymph nodes as well as in liver, spleen, lung, and kidney. However, in vitro toxicity tests, clinical data, and morphometry-assisted histopathological analyses yielded no evidence of any toxic or otherwise adverse local or systemic effects of CMS, nor did they affect the severity or course of atopic dermatitis.

Taken together, CMS accumulate in the stratum corneum in both healthy and inflammatory skin and appear to be highly biocompatible in the mouse even under conditions of atopic dermatitis and thus could potentially serve to create a depot for anti-inflammatory drugs in the skin.
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Fachbereich/EinrichtungFB Veterinärmedizin
Arbeitsbereich/InstitutInstitut für Tierpathologie
Dokumententyp/-SammlungenWissenschaftlicher Artikel
RechteCreative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Anmerkungen des AutorsGefördert durch die DFG und den Open-Access-Publikationsfonds der Freien Universität Berlin.
Erstellt am20.02.2017 - 08:46:10
Letzte Änderung20.02.2017 - 08:53:06
Statische URLhttp://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000026368