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Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection
Meresta, Anna ;  Folkert, Justyna ;  Gaber, Timo ;  Miksch, Korneliusz ;  Buttgereit, Frank ;  Detert, Jacqueline ;  Pischon, Nicole ;  Gurzawska, Katarzyna

HaupttitelPlant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection
AutorMeresta, Anna
AutorFolkert, Justyna
AutorGaber, Timo
AutorMiksch, Korneliusz
AutorButtgereit, Frank
AutorDetert, Jacqueline
AutorPischon, Nicole
AutorGurzawska, Katarzyna
Seitenzahl13 S.
Freie Schlagwörternanocoatings; Rhamnogalacturonan-I; Porphyromonas gingivalis; osteoblasts; inflammation
DDC610 Medizin und Gesundheit
Auch erschienen inInternational Journal of Nanomedicine. - 2017 (2017), 12, S. 433-445
ZusammenfassungBackground: Bioengineered plant-derived Rhamnogalacturonan-Is (RG-Is) from pectins are potential candidates for surface nanocoating of medical devices. It has recently been reported that RG-I nanocoatings may prevent bacterial infection and improve the biocompatibility of implants. The aim of the study was to evaluate in vitro impact of bioengineered RG-I nanocoatings on osteogenic capacity and proinflammatory cytokine response of murine osteoblasts following Porphyromonas gingivalis infection.
Methods: Murine MC3T3-E1 osteoblasts and isolated primary calvarial osteoblasts from C57BL/6J (B6J osteoblasts) mice were infected with P. gingivalis and incubated on tissue culture polystyrene plates with or without nanocoatings of unmodified RG-Is isolated from potato pulps (PU) or dearabinanated RG-Is (PA). To investigate a behavior of infected osteoblasts cultured on RG-Is cell morphology, proliferation, metabolic activity, mineralization and osteogenic and pro-inflammatory gene expression were examined.
Results: Following P. gingivalis infection, PA, but not PU, significantly promoted MC3T3-E1 and BJ6 osteoblasts proliferation, metabolic activity, and calcium deposition. Moreover, Il-1b, Il-6, TNF-α, and Rankl gene expressions were downregulated in cells cultured on PU and to a higher extent on PA as compared to the corresponding control, whereas Runx, Alpl, Col1a1, and Bglap gene expressions were upregulated vice versa.
Conclusion: Our data clearly showed that pectin RG-Is nanocoating with high content of galactan (PA) reduces the osteoblastic response to P. gingivalis infection in vitro and may, therefore, reduce a risk of inflammation especially in immunocompromised patients with rheumatoid or periodontal disorders.
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Fachbereich/EinrichtungMedizinische Fakultät Charité - Universitätsmedizin Berlin
Erscheinungsjahr2017
Dokumententyp/-SammlungenWissenschaftlicher Artikel
SpracheEnglisch
RechteCreative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License.
Anmerkungen des AutorsDer Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
Erstellt am16.02.2017 - 11:45:37
Letzte Änderung16.02.2017 - 11:47:51
 
Statische URLhttp://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000026356
DOI10.2147/IJN.S113740
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