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Dictyostelium discoideum as a Novel Host System to Study the Interaction between Phagocytes and Yeasts
Koller, Barbara ;  Schramm, Christin ;  Siebert, Susann ;  Triebel, János ;  Deland, Eric ;  Pfefferkorn, Anna M. ;  Rickerts, Volker ;  Thewes, Sascha

HaupttitelDictyostelium discoideum as a Novel Host System to Study the Interaction between Phagocytes and Yeasts
AutorKoller, Barbara
AutorSchramm, Christin
AutorSiebert, Susann
AutorTriebel, János
AutorDeland, Eric
AutorPfefferkorn, Anna M.
AutorRickerts, Volker
AutorThewes, Sascha
Seitenzahl18 S.
Freie Schlagwörterhost-pathogeninteraction; Saccharomyces; Candida; phagocytosis; virulence; autophagy; amoebae
DDC570 Biowissenschaften; Biologie
Auch erschienen inFrontiers in Microbiology 7:1665
ZusammenfassungThe social amoeba Dictyostelium discoideum is a well-established model organism to study the interaction between bacteria and phagocytes. In contrast, research using D. discoideum as a host model for fungi is rare. We describe a comprehensive study, which uses D. discoideum as a host model system to investigate the interaction with apathogenic (Saccharomyces cerevisiae) and pathogenic (Candida sp.) yeast. We show that Dictyostelium can be co-cultivated with yeasts on solid media, offering a convenient test to study the interaction between fungi and phagocytes. We demonstrate that a number of D. discoideum mutants increase (atg1−, kil1−, kil2−) or decrease (atg6−) the ability of the amoebae to predate yeast cells. On the yeast side, growth characteristics, reduced phagocytosis rate, as well as known virulence factors of C. albicans (EFG1, CPH1, HGC1, ICL1) contribute to the resistance of yeast cells against predation by the amoebae. Investigating haploid C. albicans strains, we suggest using the amoebae plate test for screening purposes after random mutagenesis. Finally, we discuss the potential of our adapted amoebae plate test to use D. discoideum for risk assessment of yeast strains.
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Fachbereich/EinrichtungFB Biologie, Chemie, Pharmazie
Arbeitsbereich/InstitutInstitut für Biologie
Erscheinungsjahr2016
Dokumententyp/-SammlungenWissenschaftlicher Artikel
SpracheEnglisch
RechteCreative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Anmerkungen des AutorsGefördert durch die DFG und den Open-Access-Publikationsfonds der Freien Universität Berlin.
Erstellt am21.11.2016 - 08:16:53
Letzte Änderung13.12.2016 - 09:19:01
 
Statische URLhttp://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000025727
DOI10.3389/fmicb.2016.01665
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