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The Role of the Equine Herpesvirus Type 1 (EHV-1) US3-Encoded Protein Kinase in Actin Reorganization and Nuclear Egress
Proft, Alexandra ;  Spiesschaert, Bart ;  Izume, Satoko ;  Taferner, Selina ;  Lehmann, Maik J. ;  Azab, Walid

HaupttitelThe Role of the Equine Herpesvirus Type 1 (EHV-1) US3-Encoded Protein Kinase in Actin Reorganization and Nuclear Egress
AutorProft, Alexandra
AutorSpiesschaert, Bart
AutorIzume, Satoko
AutorTaferner, Selina
AutorLehmann, Maik J.
AutorAzab, Walid
Seitenzahl16 S.
Freie SchlagwörterUS3; actin cytoskeleton; EHV-1; EHV-4; adhesion molecules; EM
DDC630 Landwirtschaft, Veterinärmedizin
Auch erschienen inViruses 2016, 8, 275
ZusammenfassungThe serine-threonine protein kinase encoded by US3 gene (pUS3) of alphaherpesviruses was shown to modulate actin reorganization, cell-to-cell spread, and virus egress in a number of virus species. However, the role of the US3 orthologues of equine herpesvirus type 1 and 4 (EHV-1 and EHV-4) has not yet been studied. Here, we show that US3 is not essential for virus replication in vitro. However, growth rates and plaque diameters of a US3-deleted EHV-1 and a mutant in which the catalytic active site was destroyed were significantly reduced when compared with parental and revertant viruses or a virus in which EHV-1 US3 was replaced with the corresponding EHV-4 gene. The reduced plaque sizes were consistent with accumulation of primarily enveloped virions in the perinuclear space of the US3-negative EHV-1, a phenotype that was also rescued by the EHV-4 orthologue. Furthermore, actin stress fiber disassembly was significantly more pronounced in cells infected with parental EHV-1, revertant, or the recombinant EHV-1 expressing EHV-4 US3. Finally, we observed that deletion of US3 in EHV-1 did not affect the expression of adhesion molecules on the surface of infected cells.
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Fachbereich/EinrichtungFB Veterinärmedizin
Arbeitsbereich/InstitutInstitut für Virologie
Erscheinungsjahr2016
Dokumententyp/-SammlungenWissenschaftlicher Artikel
SpracheEnglisch
RechteCreative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Anmerkungen des AutorsGefördert durch die DFG und den Open-Access-Publikationsfonds der Freien Universität Berlin.
Erstellt am15.12.2016 - 13:35:29
Letzte Änderung15.12.2016 - 13:36:52
 
Statische URLhttp://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000025704
DOI10.3390/v8100275
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