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An equine herpesvirus type 1 (EHV-1) expressing VP2 and VP5 of serotype 8 bluetongue virus (BTV-8) induces protection in a murine infection model
Ma, Guanggang ;  Eschbaumer, Michael ;  Said, Abdelrahman ;  Hoffmann, Bernd ;  Beer, Martin ;  Osterrieder, Nikolaus

Main titleAn equine herpesvirus type 1 (EHV-1) expressing VP2 and VP5 of serotype 8 bluetongue virus (BTV-8) induces protection in a murine infection model
AuthorMa, Guanggang
AuthorEschbaumer, Michael
AuthorSaid, Abdelrahman
AuthorHoffmann, Bernd
AuthorBeer, Martin
AuthorOsterrieder, Nikolaus
No. of Pages9 S.
Classification (DDC)630 Agriculture, Veterinary medicine
Also published inPLoS One. 2012; 7(4): e34425
AbstractBluetongue virus (BTV) can infect most species of domestic and wild ruminants causing substantial morbidity and mortality and, consequently, high economic losses. In 2006, an epizootic of BTV serotype 8 (BTV-8) started in northern Europe that caused significant disease in cattle and sheep before comprehensive vaccination was introduced two years later. Here, we evaluate the potential of equine herpesvirus type 1 (EHV-1), an alphaherpesvirus, as a novel vectored DIVA (differentiating infected from vaccinated animals) vaccine expressing VP2 of BTV-8 alone or in combination with VP5. The EHV-1
recombinant viruses stably expressed the transgenes and grew with kinetics that were identical to those of parental virus in vitro. After immunization of mice, a BTV-8-specific neutralizing antibody response was elicited. In a challenge experiment using a lethal dose of BTV-8, 100% of interferon-receptor-deficient (IFNAR2/2) mice vaccinated with the recombinant EHV-1 carrying both VP2 and VP5, but not VP2 alone, survived. VP7 was not included in the vectored vaccines and was successfully used as a DIVA marker. In summary, we show that EHV-1 expressing BTV-8 VP2 and VP5 is capable of eliciting a protective immune response that is distinguishable from that after infection and as such may be an alternative for BTV vaccination strategies in which DIVA compatibility is of importance.
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FU DepartmentDepartment of Veterinary Medicine
Other affiliation(s)Institut für Virologie
Year of publication2012
Type of documentScience article
LanguageEnglish
Terms of use/Rights Creative Commons License
Dieses Werk ist unter einer Creative Commons-Lizenz lizenziert.
Authors commentsGefördert durch die Deutsche Forschungsgemeinschaft und den Open-Access-Publikationsfonds der Freien Universität Berlin.
Created at2012-11-15 : 03:23:31
Last changed2014-01-10 : 10:32:50
 
Static URLhttp://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000015315
DOI10.1371/journal.pone.0034425
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