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An equine herpesvirus type 1 (EHV-1) expressing VP2 and VP5 of serotype 8 bluetongue virus (BTV-8) induces protection in a murine infection model
Ma, Guanggang ;  Eschbaumer, Michael ;  Said, Abdelrahman ;  Hoffmann, Bernd ;  Beer, Martin ;  Osterrieder, Nikolaus

HaupttitelAn equine herpesvirus type 1 (EHV-1) expressing VP2 and VP5 of serotype 8 bluetongue virus (BTV-8) induces protection in a murine infection model
AutorMa, Guanggang
AutorEschbaumer, Michael
AutorSaid, Abdelrahman
AutorHoffmann, Bernd
AutorBeer, Martin
AutorOsterrieder, Nikolaus
Seitenzahl9 S.
DDC630 Landwirtschaft, Veterinärmedizin
Auch erschienen inPLoS One. 2012; 7(4): e34425
ZusammenfassungBluetongue virus (BTV) can infect most species of domestic and wild ruminants causing substantial morbidity and mortality and, consequently, high economic losses. In 2006, an epizootic of BTV serotype 8 (BTV-8) started in northern Europe that caused significant disease in cattle and sheep before comprehensive vaccination was introduced two years later. Here, we evaluate the potential of equine herpesvirus type 1 (EHV-1), an alphaherpesvirus, as a novel vectored DIVA (differentiating infected from vaccinated animals) vaccine expressing VP2 of BTV-8 alone or in combination with VP5. The EHV-1
recombinant viruses stably expressed the transgenes and grew with kinetics that were identical to those of parental virus in vitro. After immunization of mice, a BTV-8-specific neutralizing antibody response was elicited. In a challenge experiment using a lethal dose of BTV-8, 100% of interferon-receptor-deficient (IFNAR2/2) mice vaccinated with the recombinant EHV-1 carrying both VP2 and VP5, but not VP2 alone, survived. VP7 was not included in the vectored vaccines and was successfully used as a DIVA marker. In summary, we show that EHV-1 expressing BTV-8 VP2 and VP5 is capable of eliciting a protective immune response that is distinguishable from that after infection and as such may be an alternative for BTV vaccination strategies in which DIVA compatibility is of importance.
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Fachbereich/EinrichtungFB Veterinärmedizin
Arbeitsbereich/InstitutInstitut für Virologie
Erscheinungsjahr2012
Dokumententyp/-SammlungenWissenschaftlicher Artikel
SpracheEnglisch
Rechte Creative Commons License
Dieses Werk ist unter einer Creative Commons-Lizenz lizenziert.
Anmerkungen des AutorsGefördert durch die Deutsche Forschungsgemeinschaft und den Open-Access-Publikationsfonds der Freien Universität Berlin.
Erstellt am15.11.2012 - 15:23:31
Letzte Änderung10.01.2014 - 10:32:50
 
Statische URLhttp://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000015315
DOI10.1371/journal.pone.0034425
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