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| An equine herpesvirus type 1 (EHV-1) expressing VP2 and VP5 of serotype 8 bluetongue virus (BTV-8) induces protection in a murine infection model Ma, Guanggang |
| Haupttitel | An equine herpesvirus type 1 (EHV-1) expressing VP2 and VP5 of serotype 8 bluetongue virus (BTV-8) induces protection in a murine infection model |
| Autor | Ma, Guanggang; Eschbaumer, Michael; Said, Abdelrahman; Hoffmann, Bernd; Beer, Martin; Osterrieder, Nikolaus |
| Seitenzahl | 9 S. |
| Auch erschienen in | PLoS One. 2012; 7(4): e34425 |
| URL des Originaldokuments | URL >> |
| Fachbereich/Einrichtung | FB Veterinärmedizin |
| Arbeitsbereich/Institut | Institut für Virologie |
| Erscheinungsjahr | 2012 |
| Dokumente | pdf-Datei
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| DDC | 630 Landwirtschaft, Veterinärmedizin |
| Dokumententyp/-Sammlungen | Aufsatz (E-Print) |
| Medientyp/Format | Text |
| Abstract | Bluetongue virus (BTV) can infect most species of domestic and wild ruminants causing substantial morbidity and mortality and, consequently, high economic losses. In 2006, an epizootic of BTV serotype 8 (BTV-8) started in northern Europe that caused significant disease in cattle and sheep before comprehensive vaccination was introduced two years later. Here, we evaluate the potential of equine herpesvirus type 1 (EHV-1), an alphaherpesvirus, as a novel vectored DIVA (differentiating infected from vaccinated animals) vaccine expressing VP2 of BTV-8 alone or in combination with VP5. The EHV-1 recombinant viruses stably expressed the transgenes and grew with kinetics that were identical to those of parental virus in vitro. After immunization of mice, a BTV-8-specific neutralizing antibody response was elicited. In a challenge experiment using a lethal dose of BTV-8, 100% of interferon-receptor-deficient (IFNAR2/2) mice vaccinated with the recombinant EHV-1 carrying both VP2 and VP5, but not VP2 alone, survived. VP7 was not included in the vectored vaccines and was successfully used as a DIVA marker. In summary, we show that EHV-1 expressing BTV-8 VP2 and VP5 is capable of eliciting a protective immune response that is distinguishable from that after infection and as such may be an alternative for BTV vaccination strategies in which DIVA compatibility is of importance. |
| Sprache | Englisch |
| Rechte |
Dieses Werk ist unter einer Creative Commons-Lizenz lizenziert. |
| Anmerkungen des Autors | Gefördert durch die Deutsche Forschungsgemeinschaft und den Open-Access-Publikationsfonds der Freien Universität Berlin. |
| Identifier | 10.1371/journal.pone.0034425 (DOI) |
| Zugriffstatistik | |
| Statische URL | http://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000015315 |
| Erstellt am | 15.11.2012 - 16:23:31 |
| Letzte Änderung | 21.11.2012 - 11:09:54 |
