Dokumentenserver der Freien Universität Berlin

Objekt-Metadaten

Establishment and characterization of a differentiated epithelial cell culture model derived from the porcine cervix uteri
Miessen, Katrin ;  Einspanier, Ralf ;  Schoen, Jennifer

Main titleEstablishment and characterization of a differentiated epithelial cell culture model derived from the porcine cervix uteri
AuthorMiessen, Katrin
AuthorEinspanier, Ralf
AuthorSchoen, Jennifer
Classification (DDC)630 Agriculture, Veterinary medicine
Also published inBMC Veterinary Research 2012, 8:31
AbstractBackground
Cervical uterine epithelial cells maintain a physiological and pathogen-free milieu in the female mammalian reproductive tract and are involved in sperm-epithelium interaction. Easily accessible, differentiated model systems of the cervical epithelium are not yet available to elucidate the underlying molecular mechanisms within these highly specialized cells. Therefore, the aim of the study was to establish a cell culture of the porcine cervical epithelium representing in vivo-like properties of the tissue.

Results
We tested different isolation methods and culture conditions and validated purity of the cultured cells by immunohistochemistry against keratins. We could reproducibly culture pure epithelial cells from cervical tissue explants. Based on a morphology score and the WST-1 Proliferation Assay, we optimized the growth medium composition. Primary porcine cervical cells performed best in conditioned Ham's F-12, containing 10% FCS, EGF and insulin. After cultivation in an air-liquid interface for three weeks, the cells showed a discontinuously multilayered phenotype. Finally, differentiation was validated via immunohistochemistry against beta catenin. Mucopolysaccharide production could be shown via alcian blue staining.

Conclusions
We provide the first suitable protocol to establish a differentiated porcine epithelial model of the cervix uteri, based on easily accessible cells using slaughterhouse material.
Documents
pdf-Datei
If your browser can't open the file, please download the file first and then open it
 
FU DepartmentDepartment of Veterinary Medicine
Other affiliation(s)Institute of Veterinary Biochemistry
Year of publication2012
Type of documentScience article
LanguageEnglish
Terms of use/Rights Creative Commons License
Dieses Werk ist unter einer Creative Commons-Lizenz lizenziert.
Authors commentsGefördert durch die DFG und den Open Access Publikationsfonds der Freien Universität Berlin
Created at2012-10-30 : 09:02:50
Last changed2014-01-10 : 10:24:24
 
Static URLhttp://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000014772
DOI10.1186/1746-6148-8-31
Statistics
 

LOADING...