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Enrichment of cancer stem cells from head and neck cancer by anchorage independent culture and related research
Chen, Chao

HaupttitelEnrichment of cancer stem cells from head and neck cancer by anchorage independent culture and related research
TitelvarianteAnreicherung von Tumorstammzellen aus Kopf-Hals-Karzinomen durch adhäsions-unabhängige Zellkultur und angewandte Forschung
AutorChen, Chao
Geburtsort: China
GutachterN.N
weitere GutachterN.N
Freie Schlagwörtercancer stem cell; head and neck cancer; epithelial mesenchymal transition
DDC610 Medizin und Gesundheit
ZusammenfassungAccumulating evidence suggests that self-renewal and differentiation capabilities reside only in a subpopulation of tumor cells, termed cancer stem cells (CSCs) that can be identified by their aldehyde-dehydrogenase-isoform-1 (ALDH1) activity. We quantified and enriched ALDH1+ cells within HNSCC cell lines by an anchorage independent culture method and subsequently characterized their phenotypic and functional properties and epithelial-to-mesenchymal transition (EMT). On patients’ tumor samples, we investigated the relationship of ALDH1 andα-smooth muscle actin (SMA) expression to metastasis of HNSCC. All cell lines formed spheroids that could self-renew and be serially re-passaged. ALDH1 expression was significantly higher in spheroid-derived cells (SDCs). ALDH1+ cells possessed self-renewal capability. SDCs had significantly higher invading activity. mRNA of the stemness-related transcription factor (TF) genes Sox2, Nanog, and Oct3/4 was significantly increased in SDCs of all cell lines. SDCs had a higher G0 phase proportion, showed high-level expression of α-SMA and Vimentin, but significantly decreased E-Cadherin expression. In 60 tumor samples from HNSCC patients, ALDH1 andα-SMA expression level was proven to closely correlate to lymph node metastasis. HNSCC-lines harbor potential CSCs, characterized by ALDH1 and stemness marker TF expression as well as properties like invasiveness, quiescence, and EMT. CSCs can be enriched by anchorage-independent culture techniques, which may be important for the investigation of their contribution to therapy resistance, tumor recurrence and metastasis.
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Fachbereich/EinrichtungMedizinische Fakultät Charité - Universitätsmedizin Berlin
Erscheinungsjahr2015
Dokumententyp/-SammlungenDissertation
Medientyp/FormatText
SpracheEnglisch
Rechte Nutzungsbedingungen
Tag der Disputation27.02.2015
Erstellt am05.03.2015 - 08:23:52
Letzte Änderung05.03.2015 - 08:27:39
 
Statische URLhttp://edocs.fu-berlin.de/diss/receive/FUDISS_thesis_000000098356
URNurn:nbn:de:kobv:188-fudissthesis000000098356-6
Zugriffsstatistik
E-Mail-Adresselancet2000@msn.com