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Human osteoblast damage after antiseptic treatment
Vörös, Pauline

HaupttitelHuman osteoblast damage after antiseptic treatment
TitelvarianteEinfluss von Antiseptika auf humane Osteoblasten in vitro
AutorVörös, Pauline
Geburtsort: Cottbus
GutachterN.N.
weitere GutachterN.N.
Freie Schlagwörterhuman osteoblasts; antiseptics; chlorhexidine; polyhexanide; periprosthetic joint infection
DDC610 Medizin und Gesundheit
ZusammenfassungPurpose. Antiseptics are a powerful medical agent used for wound treatment and decontamination and have a high potential for defeating joint infections in septic surgery. Both chlorhexidine and polyhexanide are frequently used in clinical practice and have a broad antimicrobial range, but their effect on human osteoblasts has not been sufficiently studied before. Our objective was to investigate the toxic effects of polyhexanide and chlorhexidine on human osteoblasts in vitro for evaluating their clinical applicability in septic surgery.
Methods. We isolated and cultivated human osteoblasts in vitro and assayed the toxic effects of chlorhexidine 0.1% and polyhexanide 0.04%, concentrations commonly applied in clinical practice. Toxicity was evaluated by microscopic inspection of cell morphology, trypan blue staining, evaluation of vital cell counts and determination of LDH-release.
Results. Damaged cell structure could be shown by microscopy. Both antiseptics promoted LDH activity after incubation with osteoblasts. The evaluation of vital osteoblasts showed a significant decrease of vital cells.
Conclusions. Both antiseptics induced significant cell death of osteoblasts at exposure optimum. Thus we recommend a cautious use of polyhexanide and chlorhexidine in septic surgery to avoid severe osteoblast toxicity.
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Seitenzahl17
Fachbereich/EinrichtungMedizinische Fakultät Charité - Universitätsmedizin Berlin
Erscheinungsjahr2015
Dokumententyp/-SammlungenDissertation
Medientyp/FormatText
SpracheEnglisch
Rechte Nutzungsbedingungen
Tag der Disputation27.02.2015
Erstellt am20.02.2015 - 12:39:32
Letzte Änderung20.02.2015 - 12:40:51
 
Statische URLhttp://edocs.fu-berlin.de/diss/receive/FUDISS_thesis_000000098280
URNurn:nbn:de:kobv:188-fudissthesis000000098280-3
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